Discussion part Lab Report Example | Topics and Well Written Essays - 3000 words

Discussion part - Lab Report ExampleUpon administration of satraplatin, a number of metabolites (JM118, JM383, JM518, JM559 and JM149) be formed (Raynaud et al. 1996). Platinum complexes can kill tumour cells owing to their ability to form various covalent adducts on deoxyribonucleic acid (Brabec, 2002). DNA conformation is alter by the formation of adducts which also impacts some of the other intracellular processes including DNA damage recognition by specific proteins, DNA polymerisation and repair, all of which turn over to the antitumour activity of the platinum-based compounds e.g., cisplatin (Johnson et al., 1989). Most of the chemotherapeutic drugs that have been found to be clinically valuable act by damaging DNA in proliferating cells. Thus, DNA damage is pivotal to the origin, progression and treatment of cancer. Also, higher the cytotoxicity of the compound, better the efficacy.In the present study, both the Comet assay and the MN technique were employed to assess the genotoxic set up of satraplatin and vinflunine. The comet assay essentially evaluates primary DNA damage, which is reparable, by measuring single- and/or double-strand breaks in individual cells (Collins et al., 1997). Hydrogen peroxide (H2O2) is an oxidising agent that is produced intracellularly during normal metabolism or when exposed to external agents such as solar UV radiation. H2O2 can cause DNA damage through sugar and base modifications (Dizdaroglu, 1992). In this study, humanity lymphocytes incubated for 30 min with 50M H2O2 showed substantial DNA damage, seen as a 7-fold increase in the mean Olive Tail Moment (OTM) set compared to the control sample (Fig. 1). In those cells that were subjected to a one-hour pre-incubation with satraplatin (0.02M - 200M) a significant reduction in OTM values was obtained. The observed decrease in OTM, ranging from 30% - 75%, was directly proportional to the concentration of satraplatin used. The mode of action of many